Table 1. AP parameters assessed on the three TG neuronal populations
| Parameter | MF neurones Value ± s.e.m. (n) | DMF neurones Value ± s.e.m. (n) | SS neurones Value ± s.e.m. (n) | P (MF versus DMF) | P (MF versus SS) | P (DMF versus SS) |
|---|---|---|---|---|---|---|
| The parameters considered are: Cm, membrane capacitance Vr, resting membrane potential; APampl, AP amplitude; APrt, AP repolarizing time; AHPampl, amplitude of the after-hyperpolarization; AHPdur, duration of the after-hyperpolarization at half amplitude; rd, maximal rate of depolarization; rr1, first maximal rate of repolarization; rr2, second maximal rate of repolarization; tr, time between the two maximal rate of repolarization; rheoS, minimum injected current (of 1 ms duration) that triggers an AP; rheoL, minimum injected current (of 500 ms duration) that triggers an AP; Ri, input resistance, assessed in voltage-clamp mode by applying repolarizing pulses from −60 to −80 mV. The last three columns report the significance level assessed by a t test performed on MF versus DMF, MF versus SS, and DMF versus SS neurones. P values lower than 0.05 are in bold. | ||||||
| Cm (pF) | 10.1 ± 0.5 (39) | 10.6 ± 0.4 (25) | 39.3 ± 5.8 (17) | 0.5 | 5.2e−10 | 4.9e−7 |
| Vr (mV) | −47.8 ± 0.7 (39) | −54.2 ± 0.7 (25) | −62.7 ± 0.8 (17) | 2.5e−7 | 7.4e−17 | 3.4e−9 |
| APampl (mV) | 102.1 ± 0.7 (39) | 101.6 ± 1.0 (25) | 118 ± 2 (17) | 0.66 | 8e−12 | 7.6e−9 |
| APrt (ms) | 6.1 ± 0.6 (39) | 3.2 ± 0.2 (25) | 2.1 ± 0.2 (17) | 7.5e−4 | 1.5e−4 | 5e−4 |
| AHPampl (mV) | 22.8 ± 0.5 (39) | 17.2 ± 0.6 (25) | 10.6 ± 0.7 (17) | 4.9e−10 | 8.9e−20 | 3.6e−9 |
| AHPdur (ms) | 33.4 ± 2.8 (39) | 31.4 ± 2.7 (25) | 68.4 ± 14.4 (17) | 0.63 | 0.001 | 0.004 |
| rd (V s−1) | 157 ± 9 (39) | 153 ± 9 (25) | 538 ± 46 (17) | 0.75 | 4.5e−16 | 3e−12 |
| rr1 (V s−1) | −48.0 ± 3.3 (39) | −62.7 ± 3.1 (25) | −138 ± 20 (17) | 0.003 | 4.3e−8 | 7.2e−5 |
| rr2 (V s−1) | −37.9 ± 2.1 (39) | −38.8 ± 1.9 (25) | −71.1 ± 8.9 (14) | 0.75 | 2.8e−6 | 5.4e−5 |
| tr (ms) | 5.2 ± 0.6 (39) | 2.3 ± 0.1 (25) | 1.5 ± 0.2 (14) | 3.6e−4 | 7.6e−4 | 0.004 |
| rheoS (pA pF−1) | 23.0 ± 1.8 (39) | 30.5 ± 0.9 (25) | 32.1 ± 1.9 (17) | 0.002 | 0.004 | 0.4 |
| rheoL (pA pF−1) | 3.59 ± 0.62 (39) | 5.32 ± 0.71 (25) | 12.9 ± 2.3 (17) | 0.077 | 5.6e−10 | 1.8e−6 |
| Ri (GΩ) | 1.20 ± 0.06 (39) | 0.80 ± 0.04 (25) | 0.36 ± 0.05 (17) | 1.6e−5 | 1.8e−11 | 4.2e−8 |