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Article: Local connections of excitatory neurons to corticothalamic neurons in the rat barrel cortex.
Full Text (publisher's website) ; Article Metadata ; Article Data (extracted) Tanaka YR; Tanaka YH; Konno M; Fujiyama F; Sonomura T; Okamoto-Furuta K; Kameda H; Hioki H; Furuta T; Nakamura KC; Kaneko T J. Neurosci., 2011
Table 1.
Summary of antibodies/reagents/filters used for the detection of retrograde tracers
| GFP | Cholera toxin B subunit | Fluorogold | NeuN | ||
|---|---|---|---|---|---|
| Primary antibody | |||||
| Host | Rabbit | Goat | Rabbit | Mouse | |
| Concentration | 1 μg/ml | 1:500 | 1:500 | 2 μg/ml | |
| Source | (Nakamura et al., 2008; Tamamaki et al., 2000) | List Biology | Millipore | Millipore | |
| Secondary antibody | |||||
| Target | Rabbit IgG | Goat IgG | Rabbit IgG | Mouse IgG | Mouse IgG |
| Host | Goat | Donkey | Goat | Goat | Donkey |
| Label | Biotin | Cy3 | Alexa Fluor 546 | Alexa Fluor 647 | Cy5 |
| Concentration | 10 μg/ml | 10 μg/ml | 5 μg/ml | 10 μg/ml | 10 μg/ml |
| Source | Vector | Jackson ImmunoResearch Laboratories | Invitrogen | Invitrogen | Jackson ImmunoResearch Laboratories |
| Other reagents | Streptavidin | ||||
| Label | Alexa Fluor 488 | ||||
| Concentration | 5 μg/ml | ||||
| Source | Invitrogen | ||||
| Excitation beam | 488 nm | 543 | 543 | 633 | |
| Emission filter set | 505–530 nm | 560–615 nm | 560–615 nm | >650 nm | |
Inferred neuron-electrophysiology data values
| Neuron Type | Neuron Description | Ephys Prop | Extracted Value | Standardized Value | Content Source |
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