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Article: Chronic spontaneous activity generated in the somata of primary nociceptors is associated with pain-related behavior after spinal cord injury.
Full Text (publisher's website) ; Article Metadata ; Article Data (extracted) Bedi SS; Yang Q; Crook RJ; Du J; Wu Z; Fishman HM; Grill RJ; Carlton SM; Walters ET J. Neurosci., 2010
Electrophysiological properties of all small dissociated DRG neurons
| Property | Naive | Sham | SCI | SCI vs sham | SCI vs naive | Sham vs naive |
|---|---|---|---|---|---|---|
| Soma diameter (μm) | 22.3 ± 0.5 (22, 124) | 22.4 ± 0.5 (24, 155) | 22.8 ± 0.6 (22, 163) | NS | NS | NS |
| Firing rate of neurons displaying SA (medians) (Hz) | 1.0 (12, 19) | 1.3 (15, 25) | 1.0 (23, 72) | NS | NS | NS |
| RMP (mV) | −50.3 ± 0.9 (22, 124) | −49.3 ± 0.7 (24, 157) | −49.6 ± 0.6 (23, 164) | NS | NS | NS |
| Rheobase (400 ms pulse, pA) | 72.2 ± 15.7 (22, 108) | 53.1 ± 6.1 (24, 139) | 44.3 ± 6.6 (22, 156) | NS | p < 0.01 | p < 0.05 |
| AP threshold at −50 mV (2 ms pulse, pA) | 366 ± 33 (21, 114) | 365 ± 29 (24, 148) | 298 ± 23 (22, 141) | p < 0.01 | p < 0.05 | NS |
| AP threshold at −80 mV (2 ms pulse, pA) | 760 ± 25 (21, 95) | 752 ± 23 (24, 122) | 671 ± 22 (22, 147) | p < 0.05 | p < 0.05 | NS |
| Repetitive firing (spike number, 400 ms) | 2.9 ± 0.3 (22, 116) | 3.0 ± 0.3 (24, 145) | 3.7 ± 0.3 (23, 150) | p < 0.05 | p < 0.01 | NS |
| Rm (MΩ) | 551 ± 31 (21, 117) | 591 ± 39 (24, 151) | 693 ± 44 (22, 161) | p < 0.05 | p < 0.01 | NS |
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Data are from both silent and SA neurons sampled from DRGs at all time points (3 d to 8 months) and all levels (C6/C7, T8/T9, T11/T12, and L4/L5) from both male and female animals. Except in the case of firing rate of neurons displaying SA (in which medians are presented), the numbers are means ± SEM (N of animals, N of neurons). The p values are for t test (Bonferroni corrected) applied after overall significance was established with single-factor ANOVA. Firing rates were compared with the Kruskal–Wallis test and calculated as the total number of APs in the two 30 s observation periods divided by 60 s.
Inferred neuron-electrophysiology data values
| Neuron Type | Neuron Description | Ephys Prop | Extracted Value | Standardized Value | Content Source |
|---|---|---|---|---|---|
| Dorsal root ganglion cell | resting membrane potential (mV) | -50.3 ± 0.9 | -50.3 (mV) | Data Table | |
| Dorsal root ganglion cell | cell diameter (μm) | 22.3 ± 0.5 | 22.3 (um) | Data Table | |
| Dorsal root ganglion cell | rheobase | 72.2 ± 15.7 | -- | Data Table | |
| Dorsal root ganglion cell | spike threshold | 366.0 ± 33.0 | -- | Data Table | |
| Dorsal root ganglion cell | spike threshold | 760.0 ± 25.0 | -- | Data Table | |
| Dorsal root ganglion cell | firing frequency | 1.0 | 1.0 (Hz) | Data Table |