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Article: Morphological features and electrophysiological properties of serotonergic and non-serotonergic projection neurons in the dorsal raphe nucleus. An intracellular recording and labeling study in rat brain slices.
Full Text (publisher's website) ; Article Metadata ; Article Data (extracted) Li YQ; Li H; Kaneko T; Mizuno N Brain Res., 2001
Table 1Electrical membrane properties of the serotonergic and non-serotonergic projection neurons in the dorsal raphe nucleusa
aStatistical significance was evaluated by using the two-tailed, unpaired U-test or Fisher’s exact probability test. *P<0.05 compared with non-serotonergic projection neurons in the DRN. **P<0.01 compared with non-serotonergic projection neurons in the DRN.bMean±S.D.cInput resistance was determined by hyperpolarizing current pulses, producing voltage shifts of 6–15 mV negative to the rest.dTime constants were determined by injecting a short (2 ms) depolarizing current pulse.eThe electrotonic length of an equivalent sealed-end cylinder was estimated by the expression πτ0/τ1−1.fNumbers of cases.gDetermined at the end of 500-ms current injection (see Fig. 6, arrows).
| Property | Serotonergic | Non-serotonergic |
| neurons (n=7) | neurons (n=8) | |
| Resting membrane potential (mV) | 68±13b | 70±10 |
| Input resistance (MΩ)c | 174±23 | 158±20 |
| Membrane time constant (τ0, ms)d | 7.4±1.4 | 5.5±1.3* |
| First time constant (τ1, ms)d | 1.3±1.2 | 1.1±0.7 |
| Electrotonic lengthe | 2.0±0.7 | 1.8±0.9 |
| Threshold for spikes (mV) | −75±11 | −70±8 |
| Spike height (mV) | 126±17 | 104±16* |
| Spike width at base (ms) | 3.6±1.2 | 0.9±0.3** |
| Spike width at half height (ms) | 1.2±0.4 | 0.6±0.2** |
| Adaptation of spike frequency | 1f | 7** |
| Depolarizing sag | 0 | 6** |
| Inward rectificationb | 0 | 4** |
| Rebound hump | 0 | 6** |
| Fast afterdepolarization | 0 | 5** |
| Slow afterdepolarization | 0 | 5** |
Inferred neuron-electrophysiology data values
| Neuron Type | Neuron Description | Ephys Prop | Extracted Value | Standardized Value | Content Source |
|---|---|---|---|---|---|
| Other | Dorsal Raphe Nucleus serotonergic projection neuron | input resistance (MΩ) | 174.0 ± 23.0 (7) | 174.0 (MΩ) | Data Table |
| Other | Dorsal Raphe Nucleus non-serotonergic projection neuron | input resistance (MΩ) | 158.0 ± 20.0 (8) | 158.0 (MΩ) | Data Table |
| Other | Dorsal Raphe Nucleus serotonergic projection neuron | membrane time constant | 7.4 ± 1.4 (7) | 7.4 (ms) | Data Table |
| Other | Dorsal Raphe Nucleus non-serotonergic projection neuron | membrane time constant | 5.5 ± 1.3 (8) | 5.5 (ms) | Data Table |
| Other | Dorsal Raphe Nucleus serotonergic projection neuron | spike threshold (mV) | -75.0 ± 11.0 (7) | -75.0 (mV) | Data Table |
| Other | Dorsal Raphe Nucleus non-serotonergic projection neuron | spike threshold (mV) | -70.0 ± 8.0 (8) | -70.0 (mV) | Data Table |
| Other | Dorsal Raphe Nucleus serotonergic projection neuron | spike amplitude (mV) | 126.0 ± 17.0 (7) | 126.0 (mV) | Data Table |
| Other | Dorsal Raphe Nucleus non-serotonergic projection neuron | spike amplitude (mV) | 104.0 ± 16.0 (8) | 104.0 (mV) | Data Table |
| Other | Dorsal Raphe Nucleus serotonergic projection neuron | spike width (ms) | 3.6 ± 1.2 (7) | 3.6 (ms) | Data Table |
| Other | Dorsal Raphe Nucleus non-serotonergic projection neuron | spike width (ms) | 0.9 ± 0.3 (8) | 0.9 (ms) | Data Table |
| Other | Dorsal Raphe Nucleus serotonergic projection neuron | spike half-width (ms) | 1.2 ± 0.4 (7) | 1.2 (ms) | Data Table |
| Other | Dorsal Raphe Nucleus non-serotonergic projection neuron | spike half-width (ms) | 0.6 ± 0.2 (8) | 0.6 (ms) | Data Table |
| Other | Dorsal Raphe Nucleus serotonergic projection neuron | resting membrane potential (mV) | 68.0 ± 13.0 (7) | -68.0 (mV) | Data Table |
| Other | Dorsal Raphe Nucleus non-serotonergic projection neuron | resting membrane potential (mV) | 70.0 ± 10.0 (8) | -70.0 (mV) | Data Table |