Table 1.
Primers and probes for real-time quantitative RT-PCR
| Gene (protein) | Primers and probes | |
|---|---|---|
| Scn1a (Nav1.1) | f.p. | 5′-AGAAACCCTTGAGCCCGAAG-3′ |
| r.p. | 5′-CACACTGATTTGACAGCACTTGAA-3′ | |
| h.p. | FAM-5′-TTGCTTACTGAAGGCTGTGCAGAG-3′-TAMRA | |
| Scn2a (Nav1.2) | f.p. | 5′-TCGTTGATGTCTCCTTGGTTAGC-3′ |
| r.p. | 5′-GGGACTTGATGGCACCAAGT-3′ | |
| h.p. | FAM-5′-AACTGCAAATGCCTTGGGCTATTCGG-3′-TAMRA | |
| Scn3a (Nav1.3) | f.p. | 5′-GCCGCTCCGAGCCTTATC-3′ |
| r.p. | 5′-GATGGAGGGAATTGCACCAA-3′ | |
| h.p. | FAM-5′-CGCTTTGAAGGCATGAGGGCTATTCGG-3′-TAMRA | |
| Scn8a (Nav1.6) | f.p. | 5′-CATCTTTGACTTTGTGGTGGTCAT-3′ |
| r.p. | 5′-CGGATAACTCGGAATAGGGTTG-3′ | |
| h.p. | FAM-5′-CCATTGTGGGAATGTTCCTGGCTG-3′-TAMRA | |
| Syp (synaptophysin) | f.p. | 5′-TCAGGACTCAACACCTCAGTGG-3′ |
| r.p. | 5′-AACACGAACCATAAGTTGCCAA-3′ | |
| h.p. | FAM-5′-TTTGGCTTCCTGAACCTGGTGCTCTG-3′-TAMRA | |
| Scn9a (Nav1.7) | f.p. | 5′-CTTCACTGTGGGTTGGAACA-3′ |
| r.p. | 5′-ACAGGGTAGGGGACACGAA-3′ | |
| Scn3b (Navβ3) | f.p. | 5′-TGCTACAGAAAGGTCTCTAAGGC-3′ |
| r.p. | 5′-GGTACCACAGAGTTCTCCTTGT-3′ | |
| Scn4b (Navβ4) | f.p. | 5′-TCCTGAAGAAGACCCGAGAG-3′ |
| r.p. | 5′-TGGCTTCTCTTCTGCCTTGG-3′ | |
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f.p., Forward primer; r.p., reverse primer; h.p., hybridization probe.