NeuroElectro Publications

Article: Differential electrophysiological properties of dopamine D1 and D2 receptor-containing striatal medium-sized spiny neurons.

Full Text (publisher's website) ; Article Metadata ; Article Data (extracted)
Cepeda C; André VM; Yamazaki I; Wu N; Kleiman-Weiner M; Levine MS
Eur. J. Neurosci., 2008

Table 1: Basic membrane properties of MSSNs in slices, table compiled from curating associated text and extracting data from bar graphs in Figure 1.

	Capacitance (pF)	Input resistance (MΩ)	time constant (ms)	RMP (mV)	AP amplitude (mV)	AP HW (ms)	AP threshold (mV)	AHP amplitude (mV)
D1-EGFP MSN (n = 40)	70.1 ± 3.3	186.7 ± 19.9	1.6 ± 0.1	−82.1 ± 1.4	86	1.13 ± .05	−48.4 ± 1.5	8.2 ± .46
D2-EGFP MSN (n = 65)	72.4 ± 2.2	181.2 ± 15.3	1.5 ± 0.1	−82.2 ± 1.2	87	1.16 ± .04	−53.0 ± 1.3	6.6 ± .87
: The passive membrane properties of D1 or D2 EGFP-positive cells in slices were determined in voltage-clamp mode using a small depolarizing step voltage command (10 mV) from a holding potential of −70 mV. Because the measurements of these properties were not significantly different regardless of the internal solution in the patch pipette, data were pooled for the two internal solutions. There were no significant differences in cell capacitance (70.1 ± 3.3 and 72.4 ± 2.2 pF), input resistance (186.7 ± 19.9 and 181.2 ± 15.3 MΩ) or time constant (1.6 ± 0.1 and 1.5 ± 0.1 ms) between D1 (n = 40) and D2 (n = 65) cells, respectively. After assessment of passive membrane properties, recordings were switched to current-clamp mode (K-gluconate in the patch pipette). All MSSNs displayed hyperpolarized resting membrane potentials (−82.1 ± 1.4 mV in D1 and −82.2 ± 1.2 mV in D2 cells). Injection of hyperpolarizing and depolarizing current pulses showed that both groups of cells displayed inward rectification, a typical feature of MSSNs (Fig. 1B, Cepeda et al., 1994). Examination of action potentials evoked with depolarizing current pulses or with continuous current injection did not reveal significant differences in amplitude, half-amplitude duration or afterhyperpolarization amplitudes between D1 and D2 cells (Fig. 1C). However, the threshold membrane potential for inducing action potentials was more depolarized in D1 than in D2 neurons (−48.4 ± 1.5 and −53.0 ± 1.3 mV, respectively, P

Inferred neuron-electrophysiology data values

Neuron Type	Neuron Description	Ephys Prop	Extracted Value	Standardized Value	Content Source
Neostriatum medium spiny neuron	Neostriatum Drd2-expressing, indirect projecting, medium spiny neuron	cell capacitance (pF)	72.4 ± 2.2 (65)	72.4 (pF)	Data Table
Neostriatum medium spiny neuron	Neostriatum Drd2-expressing, indirect projecting, medium spiny neuron	input resistance (MΩ)	181.2 ± 15.3 (65)	181.2 (MΩ)	Data Table
Neostriatum medium spiny neuron	Neostriatum Drd2-expressing, indirect projecting, medium spiny neuron	membrane time constant (ms)	1.5 ± 0.1 (65)	1.5 (ms)	Data Table
Neostriatum medium spiny neuron	Neostriatum Drd2-expressing, indirect projecting, medium spiny neuron	resting membrane potential (mV)	-82.2 ± 1.2 (65)	-82.2 (mV)	Data Table
Neostriatum medium spiny neuron	Neostriatum Drd2-expressing, indirect projecting, medium spiny neuron	spike amplitude (mV)	87.0 (65)	87.0 (mV)	Data Table
Neostriatum medium spiny neuron	Neostriatum Drd2-expressing, indirect projecting, medium spiny neuron	spike half-width (ms)	1.16 ± 0.04 (65)	1.16 (ms)	Data Table
Neostriatum medium spiny neuron	Neostriatum Drd2-expressing, indirect projecting, medium spiny neuron	spike threshold (mV)	-53.0 ± 1.3 (65)	-53.0 (mV)	Data Table
Neostriatum medium spiny neuron	Neostriatum Drd2-expressing, indirect projecting, medium spiny neuron	AHP amplitude (mV)	6.6 ± 0.87 (65)	6.6 (mV)	Data Table
Neostriatum medium spiny neuron	Neostriatum Drd1-expressing, direct projecting, medium spiny neuron	cell capacitance (pF)	70.1 ± 3.3 (40)	70.1 (pF)	Data Table
Neostriatum medium spiny neuron	Neostriatum Drd1-expressing, direct projecting, medium spiny neuron	input resistance (MΩ)	186.7 ± 19.9 (40)	186.7 (MΩ)	Data Table
Neostriatum medium spiny neuron	Neostriatum Drd1-expressing, direct projecting, medium spiny neuron	membrane time constant (ms)	1.6 ± 0.1 (40)	1.6 (ms)	Data Table
Neostriatum medium spiny neuron	Neostriatum Drd1-expressing, direct projecting, medium spiny neuron	resting membrane potential (mV)	-82.1 ± 1.4 (40)	-82.1 (mV)	Data Table
Neostriatum medium spiny neuron	Neostriatum Drd1-expressing, direct projecting, medium spiny neuron	spike amplitude (mV)	86.0 (40)	86.0 (mV)	Data Table
Neostriatum medium spiny neuron	Neostriatum Drd1-expressing, direct projecting, medium spiny neuron	spike half-width (ms)	1.13 ± 0.05 (40)	1.13 (ms)	Data Table
Neostriatum medium spiny neuron	Neostriatum Drd1-expressing, direct projecting, medium spiny neuron	spike threshold (mV)	-48.4 ± 1.5 (40)	-48.4 (mV)	Data Table
Neostriatum medium spiny neuron	Neostriatum Drd1-expressing, direct projecting, medium spiny neuron	AHP amplitude (mV)	8.2 ± 0.46 (40)	8.2 (mV)	Data Table