AboutNeuron TypesElectrophysiology PropertiesArticlesFAQsData/APIGet Involved

Article: Area-specific development of distinct projection neuron subclasses is regulated by postnatal epigenetic modifications.

Full Text (publisher's website) ; Article Metadata ; Article Data (extracted)
Harb K; Magrinelli E; Nicolas CS; Lukianets N; Frangeul L; Pietri M; Sun T; Sandoz G; Grammont F; Jabaudon D; Studer M; Alfano C
Elife, 2016


Figure 3—figure supplement 2 part C: Electrophysiological analyses of YFP-positive neurons from the Thy1-eYFP-H transgenic line.
Single Ctip2 (n = 7) Double Satb2 Ctip2 Type 1 (n = 9) Double Satb2 Ctip2 Type 2 (n = 9)
IV Analysis:
time to peak (ms) 256 +/- 3 246 +/- 2 255 +/- 2
sag (mV) 4.8 +/- 1.5 3.1 +/- 0.5 1.2 +/- 0.2
sag ratio 0.22 +/- 0.03 0.21 +/- 0.02 0.12 +/- 0.02
R peak (Mohm) 112.1 +/- 15.3 74.3 +/- 6.3 54.1 +/- 4.5
R ss (M Ohm) 86 +/- 10.4 56.7 +/- 3.8 46.4 +/- 3.4
type of typical AP single single double
First AP at threshold:
AP threshold (mV) -33.2 +/- 4.8 -42.1 +/- 1.7 -40.6 +/- 1.5
current injected (pA) 0.26 +/- 0.04 0.30 +/- 0.02 0.39 +/- 0.04
duration (ms) 1.58 +/- 0.07 1.48 +/- 0.07 1.69 +/- 0.12
amplitude (mV) 80.8 +/- 5.8 83.4 +/- 4.8 87 +/- 3.9
rise time (ms) 0.6 +/- 0.04 0.55 +/- 0.02 0.59 +/- 0.03
half width (ms) 0.85 +/- 0.03 0.77 +/- 0.03 0.83 +/- 0.04
Second AP at threshold:
duration (ms) 1.58 +/- 0.06 1.46 +/- 0.05 3.96 +/- 0.46
amplitude (mV) 80.4 +/- 6 82.8 +/- 4.7 72.9 +/- 3.1
rise time (ms) 0.61 +/- 0.04 0.557 +/- 0.02 0.64 +/- 0.07
half width (ms) 0.85 +/- 0.03 0.78 +/- 0.02 1.04 +/- 0.05
difference of amplitude (first-second; mV) 0.45 +/- 0.42 0.62 +/- 0.3 14.08 +/- 1.67
Inter-spike intervals (ISI):
Initial ISI at threshold (ms) 224 +/- 35 170 +/- 29 4.09 +/- 0.11
initial ISI at double threshold (ms) 37.1 +/- 8.3 23.7 +/- 3.2 4.12 +/- 0.15
first to last ISI ratio at double threshold 0.77 +/- 0.1 0.71 +/- 0.08 0.12 +/- 0.02
second to last ISI ratio at double threshold 1.28 +/- 0.15 1.17 +/- 0.07 1.15 +/- 0.14
AHP at threshold:
mAHP (mV) 13.7 +/- 1.4 11.4 +/- 1.1 11.9 +/- 1.3
mDAP (mV) 6.32 +/- 1.43 7.92 +/- 1.07 4.71 +/- 0.71
time mAHP (ms) 31.7 +/- 5.2 25.3 +/- 5.29 34.7 +/- 2.7
fAHP (mV) 10.23 +/- 0.7 8.49 +/- 1.27 4.78 +/- 1.23
fDAP (mV) 4.33 +/- 0.85 3.53 +/- 0.5 5.8 +/- 0.85
time fAHP (ms) 1.54 +/- 0.1 1.38 +/- 0.07 1.37 +/- 0.07
Electrophysiological analyses of YFP-positive neurons from the Thy1-eYFP-H transgenic line. (A). Immunofluorescences for GFP and biocytin performed on YFP+ cells from Thy1-eYFP-H transgenic brains. (B). Trains of action potentials (AP) triggered by a depolarizing current injected for 2 s to reach the threshold of AP (left) or twice this threshold (right) in cells expressing Ctip2 only (top) or both Ctip2 and Satb2. Scale bars: 20 mV Ð 250 ms for top panels and 20 mV Ð 50 ms for bottom panels (insets). (C). Table showing the main features analyzed in C/S+ and single Ctip2+ cells. Statistics (Mann-Whitney): a = difference between Single Ctip2 and Double Satb2 Ctip2 type 1 cells; b = difference between Single Ctip2 and Double Satb2 Ctip2 type 2; c = difference between Double Satb2 Ctip2 type 1 and type 2. Data are represented as means ± SEM. 1p

Report miscurated data

Inferred neuron-electrophysiology data values

Neuron Type Neuron Description Ephys Prop Extracted Value Standardized Value Content Source
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing single-firing thick-tufted pyramidal neuron sag amplitude (mV) 4.8 ± 1.5 (7) 4.8 (mV) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing single-firing thick-tufted pyramidal neuron medium AHP amplitude (mV) 13.7 ± 1.4 (7) 13.7 (mV) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing Satb2 expressing single-firing thick-tufted pyramidal neuron medium AHP amplitude (mV) 11.4 ± 1.1 (9) 11.4 (mV) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Satb2 expressing Ctip2 expressing double-firing thick-tufted pyramidal neuron medium AHP amplitude (mV) 11.9 ± 1.3 (9) 11.9 (mV) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing Satb2 expressing single-firing thick-tufted pyramidal neuron sag amplitude (mV) 3.1 ± 0.5 (9) 3.1 (mV) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing single-firing thick-tufted pyramidal neuron ADP amplitude (mV) 6.32 ± 1.43 (7) 6.32 (mV) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing Satb2 expressing single-firing thick-tufted pyramidal neuron ADP amplitude (mV) 7.92 ± 1.07 (9) 7.92 (mV) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Satb2 expressing Ctip2 expressing double-firing thick-tufted pyramidal neuron ADP amplitude (mV) 4.71 ± 0.71 (9) 4.71 (mV) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing single-firing thick-tufted pyramidal neuron medium AHP duration (ms) 31.7 ± 5.2 (7) 31.7 (ms) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing Satb2 expressing single-firing thick-tufted pyramidal neuron medium AHP duration (ms) 25.3 ± 5.29 (9) 25.3 (ms) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Satb2 expressing Ctip2 expressing double-firing thick-tufted pyramidal neuron medium AHP duration (ms) 34.7 ± 2.7 (9) 34.7 (ms) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing single-firing thick-tufted pyramidal neuron fast AHP amplitude (mV) 10.23 ± 0.7 (7) 10.23 (mV) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing Satb2 expressing single-firing thick-tufted pyramidal neuron fast AHP amplitude (mV) 8.49 ± 1.27 (9) 8.49 (mV) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Satb2 expressing Ctip2 expressing double-firing thick-tufted pyramidal neuron sag amplitude (mV) 1.2 ± 0.2 (9) 1.2 (mV) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Satb2 expressing Ctip2 expressing double-firing thick-tufted pyramidal neuron fast AHP amplitude (mV) 4.78 ± 1.23 (9) 4.78 (mV) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing single-firing thick-tufted pyramidal neuron fast AHP duration (ms) 1.54 ± 0.1 (7) 1.54 (ms) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing Satb2 expressing single-firing thick-tufted pyramidal neuron fast AHP duration (ms) 1.38 ± 0.07 (9) 1.38 (ms) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Satb2 expressing Ctip2 expressing double-firing thick-tufted pyramidal neuron fast AHP duration (ms) 1.37 ± 0.07 (9) 1.37 (ms) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing single-firing thick-tufted pyramidal neuron sag ratio 0.22 ± 0.03 (7) 0.22 (ratio) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing Satb2 expressing single-firing thick-tufted pyramidal neuron sag ratio 0.21 ± 0.02 (9) 0.21 (ratio) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Satb2 expressing Ctip2 expressing double-firing thick-tufted pyramidal neuron sag ratio 0.12 ± 0.02 (9) 0.12 (ratio) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing single-firing thick-tufted pyramidal neuron input resistance 86.0 ± 10.4 (7) 86.0 (MΩ) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing Satb2 expressing single-firing thick-tufted pyramidal neuron input resistance 56.7 ± 3.8 (9) 56.7 (MΩ) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Satb2 expressing Ctip2 expressing double-firing thick-tufted pyramidal neuron input resistance 46.4 ± 3.4 (9) 46.4 (MΩ) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing single-firing thick-tufted pyramidal neuron spike threshold (mV) -33.2 ± 4.8 (7) -33.2 (mV) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing Satb2 expressing single-firing thick-tufted pyramidal neuron spike threshold (mV) -42.1 ± 1.7 (9) -42.1 (mV) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Satb2 expressing Ctip2 expressing double-firing thick-tufted pyramidal neuron spike threshold (mV) -40.6 ± 1.5 (9) -40.6 (mV) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing single-firing thick-tufted pyramidal neuron rheobase (pA) 0.26 ± 0.04 (7) -- Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing Satb2 expressing single-firing thick-tufted pyramidal neuron rheobase (pA) 0.3 ± 0.02 (9) -- Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Satb2 expressing Ctip2 expressing double-firing thick-tufted pyramidal neuron rheobase (pA) 0.39 ± 0.04 (9) -- Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing single-firing thick-tufted pyramidal neuron spike width (ms) 1.58 ± 0.07 (7) 1.58 (ms) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing Satb2 expressing single-firing thick-tufted pyramidal neuron spike width (ms) 1.48 ± 0.07 (9) 1.48 (ms) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Satb2 expressing Ctip2 expressing double-firing thick-tufted pyramidal neuron spike width (ms) 1.69 ± 0.12 (9) 1.69 (ms) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing single-firing thick-tufted pyramidal neuron spike amplitude (mV) 80.8 ± 5.8 (7) 80.8 (mV) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing Satb2 expressing single-firing thick-tufted pyramidal neuron spike amplitude (mV) 83.4 ± 4.8 (9) 83.4 (mV) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Satb2 expressing Ctip2 expressing double-firing thick-tufted pyramidal neuron spike amplitude (mV) 87.0 ± 3.9 (9) 87.0 (mV) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing single-firing thick-tufted pyramidal neuron spike rise time (ms) 0.6 ± 0.04 (7) 0.6 (ms) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing Satb2 expressing single-firing thick-tufted pyramidal neuron spike rise time (ms) 0.55 ± 0.02 (9) 0.55 (ms) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Satb2 expressing Ctip2 expressing double-firing thick-tufted pyramidal neuron spike rise time (ms) 0.59 ± 0.03 (9) 0.59 (ms) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing single-firing thick-tufted pyramidal neuron spike half-width (ms) 0.85 ± 0.03 (7) 0.85 (ms) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing Satb2 expressing single-firing thick-tufted pyramidal neuron spike half-width (ms) 0.77 ± 0.03 (9) 0.77 (ms) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Satb2 expressing Ctip2 expressing double-firing thick-tufted pyramidal neuron spike half-width (ms) 0.83 ± 0.04 (9) 0.83 (ms) Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing single-firing thick-tufted pyramidal neuron adaptation ratio (at) 0.77 ± 0.1 (7) -- Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Ctip2 expressing Satb2 expressing single-firing thick-tufted pyramidal neuron adaptation ratio (at) 0.71 ± 0.08 (9) -- Data Table
Neocortex pyramidal cell layer 5-6 Somatosensory cortex layer V Satb2 expressing Ctip2 expressing double-firing thick-tufted pyramidal neuron adaptation ratio (at) 0.12 ± 0.02 (9) -- Data Table