Electrophysiological properties of large DCN neurons
| P13–16 | P6–9 | |||
|---|---|---|---|---|
| Mean | Range | Mean | Range | |
| Spontaneous | ||||
| “Resting” membrane potential, mV | −58.84 ± 1.71 | −71–−47 | −51.90 ± 1.86 | −62–−43 |
| Spike threshold, mV | −68.58 ± 2.41 | −95–−55 | −58.00 ± 2.24 | −70–−50 |
| Spontaneous spike frequency, Hz | 37.26 ± 4.85 | 11–83 | 12.28 ± 4.46 | 0–25 |
| Spike width, ms | 2.13 ± 0.33 | 1.1–2.6 | 6.76 ± 3.03 | 2.6–27.5 |
| Fast AHP amplitude, mV | 10.42 ± 0.66 | 6.9–17.1 | 14.56 ± 1.72 | 7.1–22.9 |
| Hyperpolarizing pulse responses | ||||
| R input, MΩ | 59.26 ± 2.97 | 39–80 | 52.80 ± 3.73 | 45–78 |
| Sag, % | 87.21 ± 2.15 | 71–100 | 94.20 ± 1.74 | 86–100 |
| RD duration, ms | 473.16 ± 58.45 | 114–855 | 126.40 ± 35.61 | 18–372 |
| RD number of spikes | 4.94 ± 0.93 | 2–12 | 1.34 ± 0.15 | 1–2 |
| RD peak frequency, Hz | 30.68 ± 9.60 | 4–156 | 35.50 ± 28.17 | 7–120 |
| Time to first RD spike, ms | 58.13 ± 7.53 | 23–128 | 50.20 ± 4.91 | 26–81 |
| RD first interspike interval, ms | 95.51 ± 18.67 | 6.1–280 | 106.33 ± 33.18 | 8.3–144 |
| Depolarizing pulse responses | ||||
| Slow AHP peak amplitude, mV | 3.14 ± 0.39 | 0.5–6.7 | 2.30 ± 0.55 | 1–6.35 |
| First interspike interval, ms | 18.87 ± 2.43 | 2.6–39.2 | 19.70 ± 7.36 | 5.3–26.6 |
| Peak spike frequency, Hz | 106.39 ± 21.77 | 7.5–384 | 90.25 ± 35.51 | 39–189 |
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Spontaneous measures refer to the spontaneous spiking properties of deep cerebellar nuclei (DCN) neurons. “Resting” membrane potential (RMP): average interspike membrane potential measured without injection of bias current. Spike threshold: membrane potential at which the neurons began to spontaneously fire spikes. Spontaneous spike frequency: frequency of spiking at RMP. Spike width: width of spike measured at half-maximal spike amplitude. Fast AHP amplitude: peak amplitude of afterhyperpolarization (AHP) immediately after a single spontaneous spike, measured relative to V mimmediately prior to spike onset. Hyperpolarizing pulse responses measures were made during and following a −0.5-nA current pulse given while the cell was held at a V m just below spike threshold with a continuous bias current.R input: input resistance. Sag: the amplitude of the ΔV m evoked by the hyperpolarizing pulse expressed as a percent ratio of the timepoint immediately prior to repolarization relative to the peak. Rebound depolarization (RD) duration: duration of the total depolarizing envelope immediately following the offset of the test pulse. RD number of spikes: average number of spikes elicited during an RD. RD peak frequency: derived from the shortest inter-spike interval during an RD. Time to first RD spike: delay between the offset of the hyperpolarizing test pulse and the first spike of the RD. RD first interspike interval: delay between the first 2 spikes during an RD. Depolarizing pulse measures were made during and following a +0.5-nA current pulse given while the cell was held at a V m just below spike threshold. Slow AHP peak amplitude: amplitude of the large AHP after the offset of the depolarizing pulse measured relative to V mimmediately prior to depolarizing current injection. First inter-spike interval: first interval between 2 spikes during the depolarizing test pulse. Peak spike frequency: derived from the shortest inter-spike interval during the depolarizing test pulse. n = 17 and 9 for P13–16 and P6–9, respectively.