TABLE 3.Physiological parameters of L6iL6 and L6iL4 interneurons
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Physiological Parameters | L6iL6 (9) | L6iL4 (9) | P Value | ROC Area | L6i (32) | L6e (72) | P Value | ROC Area |
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RMP, mV | –68.5 +/– 4.5 | –67.9 +/– 7.0 | 0.86 | –68.5 +/– 4.6 | –70.4 +/– 4.1 | 4.3E-02 | 0.61 | |
Input resistance, MΩ | 97.7 +/– 43.5 | 132.6 +/– 37.3 | 0.12 | 128.2 +/– 48.8 | 149.3 +/– 51.8 | 0.12 | ||
Membrane tau, ms | 7.2 +/– 1.9 | 10.7 +/– 3.9 | 0.06 | 9.6 +/– 3.5 | 14.4 +/– 4.1 | 1.2E-07 | 0.83 | |
Rheobase, pA | 310.0 +/– 122.1 | 146.2 +/– 75.4 | 7.3E-03 | 0.89 | 205.2 +/– 124.7 | 162.8 +/– 74.8 | 0.15 | |
AP1 amplitude, mV | 74.1 +/– 5.5 | 82.6 +/– 6.8 | 2.0E-02 | 0.88 | 80.9 +/– 9.3 | 101.8 +/– 7.8 | 1.1E-20 | 0.96 |
AP1 latency, ms | 17.3 +/– 7.8 | 76.6 +/– 44.9 | 4.4E-03 | 0.96 | 73.1 +/– 77.5 | 104.4 +/– 52.6 | 1.5E-05 | 0.72 |
AP1 half-width, ms | 0.37 +/– 0.06 | 0.63 +/– 0.07 | 6.2E-06 | 1.00 | 0.54 +/– 0.16 | 0.90 +/– 0.19 | 5.3E-15 | 0.93 |
AP1 rise slope, mV/ms | 67.4 +/– 18.9 | 78.3 +/– 13.5 | 0.22 | 76.9 +/– 21.9 | 102.4 +/– 20.8 | 1.9E-07 | 0.81 | |
AP1 fall slope, mV/ms | –117.8 +/– 24.8 | –79.9 +/– 17.2 | 4.1E-03 | 0.93 | –94.5 +/– 27.2 | –64.1 +/– 19.6 | 4.9E-09 | 0.82 |
AP1 threshold-absolute, mV | –35.3 +/– 6.2 | –37.6 +/– 6.2 | 0.49 | –36.4 +/– 5.5 | –31.3 +/– 4.9 | 1.1E-05 | 0.76 | |
AP1 threshold-relative, mV | 31.0 +/– 3.4 | 30.5 +/– 4.1 | 0.80 | 32.0 +/– 4.9 | 39.1 +/– 4.5 | 1.3E-10 | 0.87 | |
AP1 fAHP amplitude, mV | –22.5 +/– 4.9 | –17.2 +/– 4.8 | 0.06 | –19.7 +/– 4.3 | –11.4 +/– 4.4 | 2.6E-14 | 0.91 | |
AP1 sAHP amplitude, mV | –16.3 +/– 3.9 | |||||||
AP1 fAHP latency, ms | 1.76 +/– 0.38 | 3.21 +/– 1.02 | 3.6E-03 | 0.96 | 3.0 +/– 2.0 | 6.8 +/– 5.0 | 2.2E-07 | 0.82 |
AP1 sAHP latency, ms | 41.9 +/– 30.4 | |||||||
Max avg frequency, Hz | 220.7 +/– 37.2 | 129.1 +/– 56.6 | 3.0E-03 | 0.96 | 146.7 +/– 76.6 | 44.4 +/– 14.5 | 2.6E-11 | 0.88 |
Max 1st inst. frequency, Hz | 258.3 +/– 37.7 | 184.0 +/– 61.3 | 1.6E-02 | 0.84 | 221.8 +/– 65.4 | 187.0 +/– 71.1 | 1.1E-02 | 0.65 |
Max 2nd inst. frequency, Hz | 249.8 +/– 39.1 | 175.0 +/– 55.4 | 1.3E-02 | 0.89 | 205.4 +/– 61.0 | 99.9 +/– 54.9 | 6.2E-10 | 0.88 |
2xRheobase: avg. freq., Hz | 165.0 +/– 49.9 | 63.8 +/– 32.5 | 7.3E-04 | 0.96 | 86.2 +/– 71.1 | 26.5 +/– 6.4 | 4.1E-08 | 0.74 |
2xRheobase: 1st inst. freq., Hz | 200.8 +/– 47.6 | 88.3 +/– 22.6 | 1.1E-04 | 1.00 | 127.9 +/– 65.1 | 106.3 +/– 67.1 | 3.8E-02 | 0.62 |
2xRheobase: 2nd inst. freq., Hz | 191.0 +/– 52.7 | 83.0 +/– 30.2 | 5.1E-04 | 0.96 | 116.1 +/– 66.5 | 46.3 +/– 30.2 | 8.0E-10 | 0.87 |
2xRheobase: adaptation index | 24.6 +/– 14.1 | 29.1 +/– 23.9 | 0.67 | 33.2 +/– 23.5 | 68.8 +/– 16.7 | 4.2E-12 | 0.88 | |
2xRheobase: adaptation tau, ms | 276.8 +/– 354.4 | 73.6 +/– 52.5 | 0.16 | 153.1 +/– 257.3 | 12.9 +/– 12.1 | 7.8E-08 | 0.90 | |
IFavg fit: saturation freq, Hz | 616.2 +/– 755.1 | 457.8 +/– 425.7 | 0.69 | 477.0 +/– 608.7 | 70.7 +/– 32.9 | 5.6E-12 | 0.97 | |
IFavg fit: 1/slope | 985.2 +/– 1764.9 | 1002.6 +/– 1132.7 | 0.81 | 960.5 +/– 1589.6 | 277.2 +/– 248.1 | 2.7E-02 | 0.65 | |
IFavg fit: rheobase, pA | 327.8 +/– 148.6 | 181.3 +/– 50.8 | 1.3E-02 | 0.89 | 241.8 +/– 133.6 | 176.4 +/– 74.9 | 5.0E-02 | 0.65 |
IFinst fit: saturation freq, Hz | 397.0 +/– 132.7 | 812.3 +/– 980.2 | 0.29 | 508.6 +/– 535.1 | 476.9 +/– 644.9 | 5.9E-04 | 0.64 | |
IFinst fit: 1/slope | 301.3 +/– 222.6 | 1703.3 +/– 2401.8 | 0.06 | 813.1 +/– 1370.0 | 892.5 +/– 1668.8 | 0.06 | ||
IFinst fit: rheobase, pA | 333.8 +/– 138.7 | 163.7 +/– 45.2 | 2.7E-04 | 1.00 | 219.0 +/– 126.5 | 188.9 +/– 105.1 | 0.11 | |
IFinst fit slope/IFavg fit slope | 1.15 +/– 0.81 | 1.49 +/– 0.85 | 0.44 | 1.35 +/– 0.97 | 4.38 +/– 6.72 | 1.4E-02 | 0.55 |
A comparison of physiological properties of L6iL6 and L6iL4 neurons (left) and of all excitatory and inhibitory L6 neurons (right). P values of t-tests and ROC areas for each differing parameter (P < 0.05) are listed. Parameters differing significantly after the Bonferroni correction (P < 0.0031) are indicated in bold.