Article: Intrinsic membrane properties determine hippocampal differential firing pattern in vivo in anesthetized rats.
Full Text (publisher's website) ; Article Metadata ; Article Data (extracted) Kowalski J; Gan J; Jonas P; Pernía-Andrade AJ Hippocampus, 2016
CA3 | CA1 | GC | PCA3-CA1 | PCA1-GC | PCA3-GC | |
---|---|---|---|---|---|---|
Passive properties, sag ratio | ||||||
Vrest (mV) | -56.9 ± 0.7 (41) | -60.5 ± 1.1 (13) | -74.7 ± 0.9 (18) | 0.010* | <0.001* | <0.001* |
Input resistance (MΩ) | 186 ± 8 (41) | 77 ± 7 (13) | 122 ± 13 (18) | <0.001* | 0.002* | <0.001* |
Membrane time constant (ms) | 50 ± 4 (9) | 12 ± 1 (6) | 16 ± 1 (5) | <0.001* | 0.017* | <0.001* |
Sag ratio (%) | 1.7 ± 0.2 (11) | 1.6 ± 0.2 (10) | 1.0 ± 0.2 (6) | 0.557 | 0.042 | 0.015* |
Active properties - evoked firing, first AP | ||||||
AP voltage threshold (mV) | -42.3 ± 0.4 (41) | -48.4 ± 0.5 (13) | -40.8 ± 0.6 (18) | <0.001* | <0.001* | 0.034* |
AP current threshold (pA) | 18 ± 3 (36) | 20 ± 7 (13) | 135 ± 11 (18) | 0.643 | <0.001* | <0.001* |
AP amplitude (mV) | 62 ± 1 (41) | 76 ± 3 (13) | 67 ± 2 (18) | <0.001* | 0.020* | 0.170* |
AP half-duration (ms) | 0.72 ± 0.02 (41) | 0.74 ± 0.03 (13) | 0.55 ± 0.02 (18) | 0.659 | <0.001* | <0.001* |
AP max. slope of rise (V s-1) | 287 ± 11 (41) | 500 ± 44 (13) | 378 ± 29 (18) | <0.001* | 0.047* | 0.007* |
fast AHP (mV) | 3.1 ± 0.4 (6)a | -a | 7.6 ± 0.6 (18) | - | - | 0.002* |
ADP (mV) | 1.2 ± 0.4 (3)a | -a | 5.1 ± 0.7 (7)a | - | - | 0.033* |
Active properties - evoked firing, repetitive spiking properties | ||||||
Gain (Hz pA-1) | 10 ± 1 (36) | 12 ± 1 (13) | 53 ± 4 (18) | 0.272 | <0.001* | <0.001* |
Spike frequency (Hz)b | 26.2 ± 1.8 (34) | 32 ± 2 (9) | 24.5 ± 6 (17) | 0.123 | 0.068 | 0.223 |
CV of ISIb | 1.40 (34) | 1.47 (9) | 1.15 (13) | 0.714 | 0.110 | 0.132 |
Combined AP & burst frequency (Hz)b | 7.9 ± 0.5 (34) | 10.6 ± 0.9 (9) | 19.2 ± 5 (17) | 0.022* | 0.481 | 0.092 |
Spikes per burstb | 4.18 ± 0.34 (34) | 3 ± 0.24 (9) | 2.64 ± 0.39 (11)c | 0.182 | 0.102 | 0.007* |
ISI within burst (ms)b | 7.8 ± 0.57 (34) | 5.1 ± 0.34 (9) | 4.06 ± 0.13 (11)c | 0.016* | 0.020* | <0.001* |
Spontaneous action potentials and bursts | ||||||
Total AP frequency (Hz) | 3.4 ± 0.9 (9) | 6.4 ± 1.1 (5) | 0 (6) | 0.083 | 0.008* | <0.001* |
Single AP frequency (Hz) | 0.04 ± 0.02 (9) | 0.05 ± 0.01 (5) | 0 (6) | 0.17 | 0.002* | 0.024* |
Burst frequency (Hz) | 0.7 ± 0.2 (9) | 1.4 ± 0.3 (5) | 0 (6) | 0.147 | 0.002* | <0.001* |
Proportion of bursts (%) | 86.5 ± 2.4 (34) | 96.3 ± 0.8 (5) | - | 0.275 | - | - |
ISI within burst (ms) | 4.9 ± 0.2 (34) | 4.7 ± 0.3 (5) | - | 0.97 | - | - |
Spikes per burst | 6.4 ± 0.4 (34) | 4.2 ± 0.4 (5) | - | 0.024* | - | - |
Interburst interval (s) | 0.76 ± 0.17 (9) | 0.27 ± 0.05 (5) | - | 0.042* | - | - |
Interburst interval distribution τ1 (ms) | 711 ± 10 (9) | 237 ± 8 (5) | - | - | - | - |
Interburst interval distribution A1 (%) | 75 ± 1 (9) | 65 ± 2 (5) | - | - | - | - |
Interburst interval distribution τ2 (s) | 3.1 ± 0.1 (9) | 1.28 ± 0.05 (5) | - | - | - | - |
Interburst interval distribution A2 (%) | 25 ± 1 (9) | 34 ± 2 (5) | - | - | - | - |
Single AP voltage threshold (mV) | -43.9 ± 0.6 (7) | -47.2 ± 2.3 (3) | - | - | - | - |
Single AP peak amplitude (mV) | 61 ± 3 (7) | 53 ± 1 (3) | - | - | - | - |
Single AP half-duration (ms) | 0.74 ± 0.05 (7) | 0.86 ± 0.02 (3) | - | - | - | - |
Single AP max. slope of rise (V s-1) | 282 ± 27 (7) | 235 ± 15 (3) | - | - | - | - |
ADP (mV) | 0.29 ± 0.12 (7) | 0.22 ± 0.12 (3) | - | - | - | - |
APburst voltage threshold (mV) | -44.5 ± 0.6 (9) | -49.7 ± 1.3 (5) | - | 0.004* | - | - |
APburst peak amplitude (mV) | 61 ± 3 (9) | 61 ± 3 (5) | - | >0.9 | - | - |
APburst half-duration (ms) | 0.75 ± 0.04 (9) | 0.82 ± 0.04 (5) | - | 0.35 | - | - |
APburst max. slope of rise (V s-1) | 267 ± 28 (9) | 322 ± 47 (5) | - | 0.438 | - | - |
Spontaneous EPSPs and IPSPs | ||||||
EPSP frequency (Hz) | 16.8 ± 2.3 (6) | 14.2 ± 1.3 (5) | 24.1 ± 5.3 (5) | 0.662 | 0.222 | 0.247 |
EPSP peak amplitude (mV) | 1.2 ± 0.1 (6) | 1.4 ± 0.1 (5) | 1.3 ± 0.2 (5) | 0.178 | 0.548 | 0.662 |
EPSP 20 - 80% rise time (ms) | 3.1 ± 0.1 (6) | 3.5 ± 0.1 (5) | 2.1 ± 0.1 (5) | 0.017* | 0.008* | 0.004* |
EPSP τdecay (ms) | 10.7 ± 1.6 (6) | 8.4 ± 0.7 (5) | 6.2 ± 0.6 (5) | 0.247 | 0.056 | 0.009* |
Time-averaged EPSP amplitude (mV) | 1.3 ± 0.1 (6) | 1.5 ± 0.1 (5) | 1.9 ± 0.5 (5) | 0.662 | 0.691 | 0.662 |
Time-averaged IPSP amplitude (mV) | 0.48 ± 0.10 (6) | 0.41 ± 0.05 (5) | 0.07 ± 0.01 (5) | >0.9 | 0.008* | 0.004* |