Table 2.
Active and passive properties of CA1 pyramidal neurons
| Experimental condition | ||||
|---|---|---|---|---|
| No spermine | 1 mm spermine | |||
| sham-control (n = 9) | Pilocarpine-treated (n = 10) | sham-control (n = 5) | Pilocarpine-treated (n = 8) | |
| Passive properties | ||||
| Resting membrane potential (mV) | -75 ± 1 | -72 ± 1 | -72 ± 1 | -72 ± 1 |
| Membrane resistance (mΩ) | 113 ± 8 | 88 ± 11 | 111 ± 9 | 97 ± 8 |
| Membrane time constant (ms) | 28 ± 3 | 26 ± 4 | 28 ± 4 | 25 ± 3 |
| Action potential properties | ||||
| Threshold (mV) | -56 ± 1 | -52 ± 1 | -54 ± 1 | -54 ± 1 |
| Peak amplitude (mV) | 32 ± 2 | 29 ± 1 | 29 ± 1 | 27 ± 1 |
| Duration (ms) | 1.14 ± 0.05 | 1.12 ± 0.03 | 1.08 ± 0.03 | 1.06 ± 0.02 |
| Fast afterhyperpolarization (mV) | -49 ± 1 | -46 ± 1* | -50 ± 1 | -50 ± 1 |
| Peak depolarization rate (V/s) | 322 ± 15 | 267 ± 10# | 324 ± 20 | 296 ± 8 |
| Peak repolarization rate (V/s) | 77 ± 3 | 71 ± 2 | 71 ± 3 | 73 ± 2 |
| Action potential frequency (Hz) at intermediate current injection (300 pA, 200 ms) | 30.5 ± 1.3 | 28.2 ± 1.8 | 29.9 ± 3.1 | 33.2 ± 2.9 |
| Maximal action potential frequency (Hz) | 48 ± 3 | 49 ± 3 | 46 ± 5 | 46 ± 5 |
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↵*Pilocarpine-treated group significantly different from all other groups (F(3,31) = 4.78, p = 0.0075, one-way ANOVA followed by Newman-Keul's multiple comparison test with p < 0.05).
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↵#Pilocarpine-treated group significantly different from both sham groups (F(3,32) = 5.98, p = 0.0024, one-way ANOVA followed by Newman-Keul's multiple comparison test with p < 0.05).