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Article: Effects of temperature on calcium transients and Ca2+-dependent afterhyperpolarizations in neocortical pyramidal neurons.

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Lee JC; Callaway JC; Foehring RC
J. Neurophysiol., 2005



TABLE 1.Input resistance (RN) and action potential (AP) parameters


RT 32–34°C Q10

RMP, mV –71 ± 5 (17) –72 ± 3 (19) 1.03 ± 0.08
RN, MΩ 389 ± 151 (18) * 254 ± 96 (16) 0.60 ± 0.33
AP threshold, mV –39 ± 4 (20) –38 ± 4 (20) 0.98 ± 0.18
AP amplitude, mV 90 ± 11 (20) 76 ± 8 (20) 0.84 ± 0.15
AP 1/2 width, ms 2.7 ± 0.4 (20)* 1.4 ± 0.3 (20) 0.45 ± 0.1
AP base width, ms 5.25 ± 1.1 (20) * 2.9 ± 0.7 (20) 0.5 ± 0.19
dV/dt rise (V/s) 100 ± 30 (20) * 149 ± 37 (20) 1.8 ± 0.7
dV/dt repolarization (V/s) 32.5 ± 12.5 (20) * 44 ± 11 (20) 1.8 ± 0.9

Means ± SD with number of cells in parentheses; RN was calculated as RN = V/I, where V = voltage (mV) and I = current (pA). Current (I) was chosen to elicit a 10–20 mV hyperpolarization. RMP, resting membrane potential; AP 1/2 width, width of AP at point mid-way between RMP and peak of AP; AP base width, width of AP at RMP;

* , significant difference between temperatures (P < 0.05, unpaired t-test). RT (room temperature) = 23 ± 1 °C.


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Inferred neuron-electrophysiology data values

Neuron Type Neuron Description Ephys Prop Extracted Value Standardized Value Content Source
Neocortex pyramidal cell layer 2-3 Somatosensory and motor cortex layer 2-3 regular spiking pyramidal neurons resting membrane potential (mV) -72.0 ± 3.0 (19) -72.0 (mV) Data Table
Neocortex pyramidal cell layer 2-3 Somatosensory and motor cortex layer 2-3 regular spiking pyramidal neurons input resistance (MΩ) 389.0 ± 151.0 (18) 389.0 (MΩ) Data Table
Neocortex pyramidal cell layer 2-3 Somatosensory and motor cortex layer 2-3 regular spiking pyramidal neurons input resistance (MΩ) 254.0 ± 96.0 (16) 254.0 (MΩ) Data Table
Neocortex pyramidal cell layer 2-3 Somatosensory and motor cortex layer 2-3 regular spiking pyramidal neurons spike threshold (mV) -39.0 ± 4.0 (20) -39.0 (mV) Data Table
Neocortex pyramidal cell layer 2-3 Somatosensory and motor cortex layer 2-3 regular spiking pyramidal neurons spike threshold (mV) -38.0 ± 4.0 (20) -38.0 (mV) Data Table
Neocortex pyramidal cell layer 2-3 Somatosensory and motor cortex layer 2-3 regular spiking pyramidal neurons spike amplitude (mV) 90.0 ± 11.0 (20) 90.0 (mV) Data Table
Neocortex pyramidal cell layer 2-3 Somatosensory and motor cortex layer 2-3 regular spiking pyramidal neurons spike amplitude (mV) 76.0 ± 8.0 (20) 76.0 (mV) Data Table
Neocortex pyramidal cell layer 2-3 Somatosensory and motor cortex layer 2-3 regular spiking pyramidal neurons spike half-width (ms) 2.7 ± 0.4 (20) 2.7 (ms) Data Table
Neocortex pyramidal cell layer 2-3 Somatosensory and motor cortex layer 2-3 regular spiking pyramidal neurons spike half-width (ms) 1.4 ± 0.3 (20) 1.4 (ms) Data Table
Neocortex pyramidal cell layer 2-3 Somatosensory and motor cortex layer 2-3 regular spiking pyramidal neurons spike width (ms) 5.25 ± 1.1 (20) 5.25 (ms) Data Table
Neocortex pyramidal cell layer 2-3 Somatosensory and motor cortex layer 2-3 regular spiking pyramidal neurons spike width (ms) 2.9 ± 0.7 (20) 2.9 (ms) Data Table
Neocortex pyramidal cell layer 2-3 Somatosensory and motor cortex layer 2-3 regular spiking pyramidal neurons spike max rise slope (V/s) 100.0 ± 30.0 (20) 100.0 (mV/ms) Data Table
Neocortex pyramidal cell layer 2-3 Somatosensory and motor cortex layer 2-3 regular spiking pyramidal neurons spike max rise slope (V/s) 149.0 ± 37.0 (20) 149.0 (mV/ms) Data Table
Neocortex pyramidal cell layer 2-3 Somatosensory and motor cortex layer 2-3 regular spiking pyramidal neurons spike max decay slope (V/s) 32.5 ± 12.5 (20) 32.5 (mV/ms) Data Table
Neocortex pyramidal cell layer 2-3 Somatosensory and motor cortex layer 2-3 regular spiking pyramidal neurons spike max decay slope (V/s) 44.0 ± 11.0 (20) 44.0 (mV/ms) Data Table
Neocortex pyramidal cell layer 2-3 Somatosensory and motor cortex layer 2-3 regular spiking pyramidal neurons resting membrane potential (mV) -71.0 ± 5.0 (17) -71.0 (mV) Data Table