|
Article: The nitric oxide-guanylyl cyclase signaling pathway modulates membrane activity States and electrophysiological properties of striatal medium spiny neurons recorded in vivo.
Full Text (publisher's website) ; Article Metadata ; Article Data (extracted) West AR; Grace AA J. Neurosci., 2004
Effects of intrastriatal infusions of a NO scavenger alone and in combination with 8-Br-cGMP on the membrane properties of striatal neurons
| Membrane properties | Control | CPT-10 | CPT-10 + 8-Br-cGMP |
|---|---|---|---|
| Firing rate (Hz) | 0.05 ± 0.03 | 0.07 ± 0.07 | 0.44 ± 0.34 |
| Average MP (mV) | -79.3 ± 1.6 | -79.1 ± 2.0 | -72.8 ± 1.9* |
| (p < 0.05) | |||
| Up-state frequency (Hz) | 0.96 ± 0.04 | 0.95 ± 0.05 | 0.96 ± 0.08 |
| Maximal depolarized MPa (mV) | -54.8 ± 3.6 | -64.1 ± 3.0 | -53.8 ± 5.3 |
| Up-state mode (mV) | -75.8 ± 2.1 | -77.3 ± 2.1 | -69.4 ± 2.6 |
| Down-state mode (mV) | -84.9 ± 1.8 | -82.8 ± 1.7 | ±75.8 ± 1.9* |
| (p < 0.05) | |||
| Maximal hyperpolarized MP (mV) | -89.7 ± 1.5 | -87.4 ± 1.9 | -81.0 ± 1.7* |
| (p < 0.05) | |||
| Input resistance (mΩ) | 28.3 ± 2.1 | 20.3 ± 2.5* | 32.7 ± 5.6 |
| (p < 0.05) | |||
| Action potential | |||
| Amplitude (mV) | 64.1 ± 1.9 | 67.4 ± 2.2 | 58.5 ± 3.2 |
| Overshoot (mV) | +20.7 ± 2.9 | +18.6 ± 3.2 | +13.4 ± 3.6 |
| Duration (msec) | 1.1 ± 0.04 | 1.0 ± 0.04 | 1.2 ± 0.10 |
| Threshold (mV) | -43.4 ± 1.7 | -48.6 ± 2.1 | -44.8 ± 3.3 |
| AHP (mV) | -14.0 ± 1.1 | -14.2 ± 0.8 | -10.2 ± 1.5 |
-
All data are means ± SEM from n = 8-11 neurons. MP, Membrane potential; AHP, afterhyperpolarization.
-
↵* Statistical significance was determined by comparing aCSF control to drug groups using an ANOVA with Dunnett's post hoc test.
-
↵ a This calculation does not include membrane potential fluctuations contributed by action potentials.