Article: The nitric oxide-guanylyl cyclase signaling pathway modulates membrane activity States and electrophysiological properties of striatal medium spiny neurons recorded in vivo.
Full Text (publisher's website) ; Article Metadata ; Article Data (extracted) West AR; Grace AA J. Neurosci., 2004
Membrane properties | Control | ODQ | ODQ+cGMP | Zaprinast |
---|---|---|---|---|
Firing rate (Hz) | 0.54 ± 0.33 | 0.06 ± 0.06 | 0.05 ± 0.04 | 0.91 ± 0.35** |
(p<0.05) | ||||
Average MP (mV) | -78.2 ± 2.7 | -84.7 ± 2.3 | -79.2 ± 2.7 | -65.0 ± 0.76* |
(p<0.05) | ||||
Up-state frequency (Hz) | 1.17 ± 0.11 | 1.03 ± 0.14 | 0.90 ± 0.06 | 0.97 ± 0.06 |
Maximal depolarized MPa (mV) | -55.0 ± 3.5 | -72.2 ± 2.5* | -55.0 ± 2.0 | -44.4 ± 2.7* |
(p<0.05) | (p<0.05) | |||
Up-state mode (mV) | -73.0 ± 4.2 | -81.4 ± 4.0 | -75.8 ± 1.8 | -61.2 ± 1.7* |
(p<0.05) | ||||
Down-state mode (mV) | -83.0 ± 3.8 | -87.4 ± 4.6 | -87.0 ± 1.4 | -72.0 ± 3.7 |
Maximal hyperpolarized MP (mV) | -91.3 ± 3.2 | -90.8 ± 3.1 | -91.8 ± 1.4 | -80.4 ± 2.9* |
(p<0.05) | ||||
Input resistance (mΩ) | 34.3 ± 1.8 | 17.7 ± 3.5* | 20.3 ± 1.0 | 28.4 ± 1.8 |
(p<0.05) | ||||
Action potential Amplitude (mV) | 70.0 ± 2.4 | 60.0 ± 2.4* | 68.2 ± 3.1 | 71.6 ± 2.5 |
(p<0.05) | ||||
Overshoot (mV) | +22.1 ± 2.8 | +14.7 ± 2.7 | +22.5 ± 1.6 | +25.9 ± 3.5 |
Duration (msec) | 1.2 ± 0.08 | 1.3 ± 0.05 | 1.1 ± 0.07 | 1.2 ± 0.05 |
Threshold (mV) | -47.5 ± 1.2 | -45.7 ± 3.0 | -45.6 ± 2.2 | -45.7 ± 1.5 |
AHP (mV) | -12.5 ± 1.6 | -12.3 ± 2.4 | -12.9 ± 0.4 | -10.9 ± 1.4 |
-
All data are means ± SEM from n = 5-6 neurons. MP, Membrane potential; AHP, afterhyperpolarization.
-
↵* Statistical significance was determined by comparing DMSO control to drug groups using an ANOVA with Dunnett's post hoc test.
-
↵** Significantly different compared to the ODQ group using an ANOVA with Dunnett's post hoc test.
-
↵ a This calculation does not include membrane potential fluctuations contributed by action potentials.