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Article: Granule-like neurons at the hilar/CA3 border after status epilepticus and their synchrony with area CA3 pyramidal cells: functional implications of seizure-induced neurogenesis.
Full Text (publisher's website) ; Article Metadata ; Article Data (extracted) Scharfman HE; Goodman JH; Sollas AL J. Neurosci., 2000
Membrane properties of granule-like neurons and granule cells in the granule cell layer of pilocarpine-treated rats and saline-treated controls
| RMP (mV) | Rin (MΩ) | τ (msec) | AP amp | AP slope | AP dur | AHP | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Total amp (mV) | From thresh. (mV) | Max. rise (V/S) | Max. decay (V/S) | dv/dt ratio (rise/decay) | Total dur. (msec) | ½ dur. (msec) | Peak amp. (mV) | Total dur. (msec) | ||||
| Pilocarpine-treated | ||||||||||||
| Granule-like neurons | ||||||||||||
| Mean | −75.8 | 58.1 | 9.25 | 105.4 | 77.2 | 401 | 75 | 5.5 | 1.64 | 0.17 | 6.3 | 5.33 |
| SEM | 1.9 | 6.5 | 4.8 | 4.4 | 2.0 | 31 | 6 | 0.4 | 0.10 | 0.01 | 1.2 | 0.99 |
| n | 6 | 8 | 8 | 8 | 8 | 8 | 8 | 8 | 8 | 8 | 6 | 6 |
| Granule cells in the granule cell layer | ||||||||||||
| Mean | −72.8 | 43.1 | 8.14 | 100.3 | 79.2 | 434 | 89 | 5.0 | 1.70 | 0.20 | 8.0 | 7.00 |
| SEM | 1.8 | 2.9 | 1.1 | 1.3 | 4.0 | 36 | 6 | 0.3 | 0.11 | 0.02 | 0.9 | 0.91 |
| n | 7 | 8 | 7 | 8 | 8 | 11 | 11 | 11 | 11 | 10 | 8 | 7 |
| Saline-treated | ||||||||||||
| Granule cells in the granule cell layer | ||||||||||||
| Mean | −71.5 | 57.5 | 11.2 | 103.6 | 74.6 | 420 | 92 | 4.8 | 1.51 | 0.19 | 7.2 | 5.20 |
| SEM | 2.9 | 7.5 | 2.4 | 7.8 | 1.9 | 55 | 9 | 0.2 | 0.07 | 0.03 | 1.3 | 0.56 |
| n | 4 | 4 | 4 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
| Granule cells 50–100 μm from the granule cell layer/hilar border | ||||||||||||
| Mean | −72.0 | 61.7 | 7.33 | 102.7 | 72.0 | 433 | 92 | 4.7 | 1.67 | 0.21 | 6.0 | 5.67 |
| SEM | 1.2 | 3.3 | 1.4 | 3.7 | 1.2 | 60 | 4 | 0.7 | 0.17 | 0.01 | 0.3 | 0.07 |
| n | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 | 3 |
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For explanation of parameters, see Materials and Methods. In pilocarpine-treated rats, granule cells in the cell layer were recorded from the same slices as those used to record granule-like neurons. Granule-like neurons were not significantly different from granule cells in the granule cell layer in any parameter listed (Student'st test, p > 0.05).